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|Tested species reactivity||Human , Rat , Mouse|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Full-length human Plk1 (polo-like kinase 1)|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Flow Cytometry (Flow)||3-5 µg x 10^6 cells|
|Immunocytochemistry (ICC)||Assay Dependent|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 2 publications below|
Considerable evidence indicates that a Polo-Like Kinase (PLK) plays an important role in cell cycle regulation. PLK is also required for bipolar spindle formation, activation of the anaphase-promoting complex/cyclosome, and cytokinesis. Recent work led to the identification of a PLKK that is thought to be responsible for activation of PLK. Recent work (Erikson, et al., 2004) has shown that PLKK is in turn activated by phosphorylation at three sites (Ser482, Ser486 and Ser490). Thus activation of PLK is thought to involve a kinase cascade involving the phosphorylation of Ser482,486,490 in PLKK.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
SAPK pathways and p53 cooperatively regulate PLK4 activity and centrosome integrity under stress.
37-7000 was used in western blot to study the regulation of polo-like kinase 4 activity and centrosome numeral integrity by SAPK pathways and p53.
|Nakamura T,Saito H,Takekawa M||Nature communications (4:null)||2013|
Three independent mechanisms for arrest in G2 after ionizing radiation.
37-7000 was used in western blot to identify and characterize radiation-induced G2-arrest in human B-lymphocyte cancer cell lines.
|Landsverk KS,Patzke S,Rein ID,Stokke C,Lyng H,De Angelis PM,Stokke T||Cell cycle (Georgetown, Tex.) (10:819)||2011|
PLK-1, cell cycle regulated protein kinase, polo (Drosophia)-like kinase, polo like kinase, serine/threonine-protein kinase 13, serine/threonine-protein kinase PLK1, polo-like kinase homolog, Serine/threonine-protein kinase PLK1, STPK13, PLK1, PLK