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|Tested species reactivity||Mouse , Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Purified recombinant fragment of human SMAD2 expressed in E. Coli.|
|Contains||0.03% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1/200 - 1/400|
|Immunofluorescence (IF)||1/200 - 1/1000|
|Immunohistochemistry (IHC)||1/200 - 1/1000|
|Western Blot (WB)||1/500 - 1/2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-15877 targets SMAD2 in FACS, IF, IHC, and WB applications and shows reactivity with Human samples.
The MA5-15877 immunogen is purified recombinant fragment of human SMAD2 expressed in E. Coli. .
MA5-15877 detects SMAD2 which has a predicted molecular weight of approximately 52kDa.
The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. This protein can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin. Alternatively spliced transcript variants encoding the same protein have been observed.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
MAD homolog 2, SMAD, mothers against DPP homolog 2, Sma- and Mad-related protein 2, mother against DPP homolog 2, mothers against decapentaplegic homolog 2, Smad 2, mad-related protein 2, mothers against DPP homolog 2, JV18, JV18-1, MADH2, MADR2, hMAD-2, hSMAD2, MAD homolog 2
hSMAD2, Madh2, JV18, MADH2, 7120426M23Rik, Madr2, MADR2, mMad2, Smad-2, JV18-1, hMAD-2
Nucleoside, nucleotide and nucleic acid metabolism mRNA transcription mRNA transcription regulation Signal transduction Cell surface receptor mediated signal transduction Receptor protein serine/threonine kinase signaling pathway Intracellular signaling cascade Other intracellular signaling cascade Cell communication Ligand-mediated signaling Oncogenesis Developmental processes