Flow cytometric analysis of HeLa human cervical epithelial carcinoma cell line was stained with Sheep Anti-human Twist-1 Fluorescein-conjugated Antigen Affinity-purified Polyclonal Antibody (Product # PA5-46889) or isotype control antibodyopen histogram. To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I.
|Tested species reactivity||Human|
|Host / Isotype||Sheep / IgG|
|Immunogen||E. coli-derived recombinant human Twist-1 Met1-His202|
|Purification||Antigen affinity chromatography|
|Storage buffer||Buffered saline solution with BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C, store in dark, DO NOT FREEZE!|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||10 µl/10^6 cells|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
In direct ELISAs, less than 16% cross-reactivity with recombinant human Twist-2 is observed.
Basic helix-loop-helix (bHLH) transcription factors have been implicated in cell lineage determination and differentiation. The protein encoded by this gene is a bHLH transcription factor and shares similarity with another bHLH transcription factor, Dermo1. The strongest expression of this mRNA is in placental tissue, in adults, mesodermally derived tissues express this mRNA preferentially. Mutations in this gene have been found in patients with Saethre-Chotzen syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.