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|Tested species reactivity||Not Applicable|
|Host / Isotype||Yeast|
|Storage buffer||0.1M citric acid|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Control (Ctrl)||Assay Dependent|
|ELISA (ELISA)||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
For protein verification, the expressed protein was N-terminal sequenced and begins with the following 10 amino acids: EAEAERLEED. The EAEA portion showed incomplete cleavage of the alpha-Factor signal by the Ste 13 protease. Several amino acids were generated from the cloning vector. Purity is >95% by SDS-PAGE analyses.
Mature porcine IL-6 was expressed in Pichia pastoris, KM71H. GenBank Accession # P26893, amino acids 30 - 212.
Activity: The ED50 (or 1 unit of activity) of recombinant porcine IL-6 is typically 45 pg/ml. For in vitro applications. A dose-response assay should be performed to determine the optimal concentration for specific experiments.
Reconstitution and Dilution: Store lyophilized material at 4°C. Reconstitute in distilled water to a concentration of 0.1 - 1 mg/ml. Dilute in a buffer-containing carrier protein, aliquot, and store at or below -70°C, avoiding multiple freeze-thaw cycles. PBS with 1% BSA or a neutral media containing <10% FCS are suitable buffers.
Endotoxin level is <0.05 EU/ug.
This gene encodes a cytokine that functions in inflammation and the maturation of B cells. The protein is primarily produced at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
IL-6, interleukin-6, interleukin-6 protein, interleukin HP-1
Signal transduction Cell surface receptor mediated signal transduction Cytokine and chemokine mediated signaling pathway Intracellular signaling cascade MAPKKK cascade JNK cascade JAK-STAT cascade Cell communication Ligand-mediated signaling Immunity and defense Apoptosis Inhibition of apoptosis