|Tested species reactivity||Rat|
|Host / Isotype||E.coli|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Control (Ctrl)||Assay Dependent|
|ELISA (ELISA)||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Purity: >98% pure by SDS-PAGE analyses.
Activity: One unit of activity equals 0.01 ng/ml, as determined in a proliferation assay with IL-6 dependent mouse 7TD1 cells.
Use: Rat IL-6 exerts activity in the range of 0.01 to 1.0 ng/ml for most in vitro applications.
Reconstitution and Dilution: Reconstitute in distilled water or buffer at pH >7 to a concentration of 100 ng/ul. After reconstitution, dilute cytokine in a neutral buffer containing carrier protein such as 5-10% FBS or 1-4% high purity BSA (fatty acid free fraction V).
Lyophilized product is stable until expiration if stored desiccated below 0°C. Cytokines diluted as indicated above should be divided into aliquots and stored at -70°C. Recombinant proteins should be stored at concentrations >500 ng/ml to reduce loss of activity.
This gene encodes a cytokine that functions in inflammation and the maturation of B cells. The protein is primarily produced at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.