Immunohistochemical analysis of confluent MDCK type 1 cells stained with Claudin 1 Polyclonal Antibody (PA1-37464). Cells were blocked with 1% BSA, 0.1% gelatin, 0.5% Triton X-100 and stained with PA1-37464 (1:4) followed by a fluorophore-conjugated anti-rabbit IgG secondary antibody to detect Claudin 1 (green). Data courtesy of the Innovators Program.
|Tested species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide derived from C-terminus of human claudin-1 peptide|
|Purification||Antigen affinity chromatography|
|Storage buffer||TBS, pH 7.6, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||Ready-to-use|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is predicted to react with bovine, canine, porcine and rat based on sequence homology.
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is skin, breast carcinoma or ovarian carcinoma.
Claudin proteins are a family of proteins associated with tight junctions. Tight junctions are specialized regions of cell to cell contact; made up of network of strands to act as a molecular gasket for preventing the leakage of ions, water etc. between cells. They are abundant in luminal epithelial sheets where they maintain epithelial cell polarity. Different tissues exhibit different Claudin composition.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.