Western blot analysis of TCR V alpha 12.1 was performed by loading 25 ug of Jurkat (lane 1), CEM (lane 2) and rat thymus (lane 3) cell lysates onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked at 4°C overnight. The membrane was probed with a TCR V alpha 12.1 monoclonal antibody (Product # TCR1764) at a dilution of 1:10 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hr at room temperature in the dark. Chemiluminescent detection was performed using Pierce ECL Plus Western Blotting Substrate (Product # 32132). Results show a band at ~30-35 kDa.
|Tested species reactivity||Human, Rat|
|Published species reactivity||Mouse, Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Human TCR V alpha 12.1|
|Storage buffer||PBS with 0.5% BSA|
|Contains||0.1% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1-2 µg/test|
|Immunohistochemistry (Frozen) (IHC (F))||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
|T-Cell Activation (TCA)||Assay Dependent|
|Western Blot (WB)||1:1-1:100|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
The ability of T cell receptors (TCR) to discriminate foreign from self-peptides presented by major histocompatibility complex (MHC) class II molecules is essential for an effective adaptive immune response. TCR recognition of self-peptides has been linked to autoimmune disease. Mutant self-peptides have been associated with tumors. Engagement of TCRs by a family of bacterial toxins know as superantigens has been responsible for toxic shock syndrome. Autoantibodies to V beta segments of T cell receptors have been isolated from patients with rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE). The autoantibodies block TH1-mediated inflammatory autodestructive reactions and are believed to be a method by which the immune system compensates for disease (ref5). T Cell and TCR Diversity Most human T cells express the TCR alpha-beta and either CD4 or CD8 molecule (single positive, SP). A small number of T cells lack both CD4 and CD8 (double negative, DN). Increased percentages of alpha-beta DN T cells have been identified in some autoimmune and immunodeficiency disorders. Gamma-delta T cells are primarily found within the epithelium. They show less TCR diversity and recognize antigens differently than alpha-beta T cells. Subsets of gamma-delta T cells have shown antitumor and immunoregulatory activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Restricted autoantigen recognition associated with deletional and adaptive regulatory mechanisms.
TCR1764 was used in flow cytometry to investigate the mechanism for autoreactive T cells in autoimmune diabetes
|Gebe JA,Yue BB,Unrath KA,Falk BA,Nepom GT||Journal of immunology (Baltimore, Md. : 1950) (183:59)||2009|
Autoreactive human T-cell receptor initiates insulitis and impaired glucose tolerance in HLA DR4 transgenic mice.
TCR1764 was used in flow cytometry to study the role of autoreactive human T-cell receptor in insulitis and impairement of glucose tolerance
|Gebe JA,Unrath KA,Yue BB,Miyake T,Falk BA,Nepom GT||Journal of autoimmunity (30:197)||2008|
Cutting edge: size and diversity of CD4+CD25high Foxp3+ regulatory T cell repertoire in humans: evidence for similarities and partial overlapping with CD4+CD25- T cells.
TCR1764 was used in flow cytometry to characterize the CD4+CD25high Foxp3+ regulatory T cell repertoire
|Fazilleau N,Bachelez H,Gougeon ML,Viguier M||Journal of immunology (Baltimore, Md. : 1950) (179:3412)||2007|
Identification of an enhancer agonist cytotoxic T lymphocyte peptide from human carcinoembryonic antigen.
TCR1764 was used in flow cytometry to identify a specific peptide from human carcinoembryonic antigen to enhance the immunogenicity of tumor cell self-antigens
|Zaremba S,Barzaga E,Zhu M,Soares N,Tsang KY,Schlom J||Cancer research (57:4570)||1997|
Clonal V alpha 12.1+ T cell expansions in the peripheral blood of rheumatoid arthritis patients.
TCR1764 was used in immunohistochemistry to investigate the increase of V alpha 12.1+, CD8+ T cells in rheumatoid arthritis patients
|DerSimonian H,Sugita M,Glass DN,Maier AL,Weinblatt ME,Rème T,Brenner MB||The Journal of experimental medicine (177:1623)||1993|