|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A 15 amino acid peptide near the carboxy terminus of human ARHGAP18.|
|Purification||Antigen affinity chromatography|
|Contains||0.02% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||2.5 µg/ml|
|Immunofluorescence (IF)||20 ug/ml|
|Western Blot (WB)||1-2 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
A suggested positive control is NIH-3T3 cell lysate.
PA5-20893 can be used with blocking peptide PEP-1007.
ARHGAP18 is one member of the human RhoGAP family with approximately 80 RhoGAP proteins known to be encoded in the human genome. Rho proteins belong to the Ras superfamily that is composed of over 50 members divided into six families, including Ras, Sar, Rho, Ran, Rab and Arf. Rho GTPases are important regulators of the actin cytoskeleton and consequently influence the shape and migration of cells. ARHGAP18 is linked to Ras, and thus, to EGFR-mediated proliferation, migration and differentiation. ARHGAP18 is precisely contained within chromosome 6q22-24, which has been shown to be linked to schizophrenia, suggesting that ARHGEP18 may play a role in this condition.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.