|Tested species reactivity||Mouse|
|Published species reactivity||Rat|
|Host / Isotype||Rat / IgG2a|
|Immunogen||Extracellular domain of murine CD40 and the Fc portion of human IgG1.|
|Storage buffer||PBS with 1% BSA, 5% sucrose|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C, store in dark, DO NOT FREEZE!|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Neat|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Reconstitute with 1 ml of distilled water. After reconstitution, store product undiluted at 4°C in the dark. Once reconstituted, this product has a shelf life of approx. 18 months. Once reconstituted, this product has a shelf life of approx. 18 months.
For FACS analysis, use 10ul of the suggested working dilution to label 1x10^6 cells in 100ul.
The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor has been found to be essential in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. AT-hook transcription factor AKNA is reported to coordinately regulate the expression of this receptor and its ligand, which may be important for homotypic cell interactions. Adaptor protein TNFR2 interacts with this receptor and serves as a mediator of the signal transduction. The interaction of this receptor and its ligand is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protease-activated receptor 1 activation is necessary for monocyte chemoattractant protein 1-dependent leukocyte recruitment in vivo.
MA1-81163 was used in immunocytochemistry to study the role of PAR activation in vivo to initiate MCP-dependent leukocyte recruitment
|Chen D,Carpenter A,Abrahams J,Chambers RC,Lechler RI,McVey JH,Dorling A||The Journal of experimental medicine (205:1739)||2008|
A transmembrane-anchored rat RAE-1-like transcript as a ligand for NKR-P2, the rat ortholog of human and mouse NKG2D.
MA1-81163 was used in flow cytometry to identify the ligand for the rat ortholog of human and mouse NKG2D
|Savithri B,Khar A||European journal of immunology (36:107)||2006|
Target cell induced activation of NK cells in vitro: cytokine production and enhancement of cytotoxic function.
MA1-81163 was used in flow cytometry to investigate the changes of cytokine expression in NK cells after interacting with tumor cells
|Das S,Varalakshmi C,Kumari AL,Patel M,Khar A||Cancer immunology, immunotherapy : CII (50:428)||2001|
Interleukin-2-induced nitric oxide synthase and nuclear factor-kappaB activity in activated natural killer cells and the production of interferon-gamma.
MA1-81163 was used in flow cytometry to investigate the role of interleukin 2 in the regulation of natural killer cell function
|Jyothi MD,Khar A||Scandinavian journal of immunology (52:148)||2000|
Target-cell-induced anergy in natural killer cells: suppression of cytotoxic function.
MA1-81163 was used in flow cytometry to investigate the cytotoxic functions of natural killer cells
|Das S,Varalakshmi C,Khar A||Cancer immunology, immunotherapy : CII (49:109)||2000|
Administration of anti-IL-12 antibody in vivo inhibits rejection of a rat histiocytoma and suppresses cytokine response in a tumour-bearing host.
MA1-81163 was used in flow cytometry to investigate the effect of interleukin 12 inhibition on tumor rejection
|Rao KL,Varalakshmi C,Ali AM,Khar A||Immunology (92:381)||1997|
Natural killer cell activation-associated induction of target cell DNA fragmentation in a spontaneously regressing rat histiocytoma.
MA1-81163 was used in flow cytometry to investigate the effect of natural killer cells on apoptosis in a regressing histiocytoma
|Bright JJ,Kausalya S,Khar A||Immunology (85:638)||1995|
Efficient suicide gene therapy of transduced and distant untransduced ovary tumors is correlated with significant increase of intratumoral T and NK cells.
MA1-81163 was used in immunohistochemistry to study the mechanism for the suicide gene therapy of ovary tumors
|Nagy HJ,Panis Y,Fabre M,Engelmann C,Soubrane O,Houssin D,Klatzmann D||Biomedicine and pharmacotherapy = Biomedecine and pharmacotherapie (54:479)||2000|
Eradication of rat malignant gliomas by retroviral-mediated, in vivo delivery of the interleukin 4 gene.
MA1-81163 was used in immunohistochemistry to evaluate the interleukin 4 gene therapy of rat malignant gliomas
|Benedetti S,Bruzzone MG,Pollo B,DiMeco F,Magrassi L,Pirola B,Cirenei N,Colombo MP,Finocchiaro G||Cancer research (59:645)||1999|
Phospholipase A2-mediated inflammation induces regression of malignant gliomas.
MA1-81163 was used in immunohistochemistry to investigate the effect of phospholipase A2 on glioma inhibition in vivo
|Goddard DH,Bomalaski JS,Lipper S,Shorr RG,Clark MA||Cancer letters (102:1)||1996|
Linomide inhibits angiogenesis, growth, metastasis, and macrophage infiltration within rat prostatic cancers.
MA1-81163 was used in immunohistochemistry to study the role of mitochondrial pathway in neutrophil survival modulated by IgE in asthma
|Vukanovic J,Isaacs JT||Cancer research (55:1499)||1995|