Flow cytometry analysis of TCR delta on TCR delta positive PEER cells (left panel) or TCR delta negative HPB-ALL cells (right panel). Equal numbers of cells were stained with a FITC labeled TCR Delta TCS1 (TS-1) monoclonal antibody (Product # TCR2055) or were left unstained (black histograms). 5ul of primary antibody were used per test. All antibody incubations were performed for 30 minutes at room temperature. A representative 10,000 cells were acquired for each sample.
|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Human TCR Vdelta1-Jdelta2|
|Storage buffer||PBS with glycerol, 0.5% BSA|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1-2 µg/test|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
TCR2055 targets TCR Delta TCS1 in FACS applications and shows reactivity with Human samples.
The TCR2055 immunogen is human TCR Vdelta1-Jdelta2.
TCR2055 detects TCR Delta TCS1 which has a predicted molecular weight of approximately 127 kDa.
Washing step with 0.1% Tween is necessary to expose antigen.
The ability of T cell receptors (TCR) to discriminate foreign from self-peptides presented by major histocompatibility complex (MHC) class II molecules is essential for an effective adaptive immune response. TCR recognition of self-peptides has been linked to autoimmune disease. Mutant self-peptides have been associated with tumors. Engagement of TCRs by a family of bacterial toxins know as superantigens has been responsible for toxic shock syndrome. Autoantibodies to V beta segments of T cell receptors have been isolated from patients with rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE). The autoantibodies block TH1-mediated inflammatory autodestructive reactions and are believed to be a method by which the immune system compensates for disease (ref5). T Cell and TCR Diversity Most human T cells express the TCR alpha-beta and either CD4 or CD8 molecule (single positive, SP). A small number of T cells lack both CD4 and CD8 (double negative, DN). Increased percentages of alpha-beta DN T cells have been identified in some autoimmune and immunodeficiency disorders. Gamma-delta T cells are primarily found within the epithelium. They show less TCR diversity and recognize antigens differently than alpha-beta T cells. Subsets of gamma-delta T cells have shown antitumor and immunoregulatory activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Human cytomegalovirus elicits fetal gammadelta T cell responses in utero.
TCR2055 was used in flow cytometry to examine the effect of human cytomegalovirus on fetal gammadelta T cell responses in utero
|Vermijlen D,Brouwer M,Donner C,Liesnard C,Tackoen M,Van Rysselberge M,Twité N,Goldman M,Marchant A,Willems F||The Journal of experimental medicine (207:807)||2010|
Gamma delta+ T cells involvement in viral immune control of chronic human herpesvirus 8 infection.
TCR2055 was used in flow cytometry to investigate the role of Gamma delta+ T cells in the immune response against chronic human herpesvirus 8 infection
|Barcy S,De Rosa SC,Vieira J,Diem K,Ikoma M,Casper C,Corey L||Journal of immunology (Baltimore, Md. : 1950) (180:3417)||2008|
Human CD1-restricted T cell recognition of lipids from pollens.
TCR2055 was used in flow cytometry to study the immune response of pollen lipids
|Agea E,Russano A,Bistoni O,Mannucci R,Nicoletti I,Corazzi L,Postle AD,De Libero G,Porcelli SA,Spinozzi F||The Journal of experimental medicine (202:295)||2005|
T cell receptor/CD3-signaling induces death by apoptosis in human T cell receptor gamma delta + T cells.
TCR2055 was used in flow cytometry to investigate the death of human peripheral blood gamma delta+ T cells induced by anti-TCR or -CD3 antibodies
|Janssen O,Wesselborg S,Heckl-Ostreicher B,Pechhold K,Bender A,Schondelmaier S,Moldenhauer G,Kabelitz D||Journal of immunology (Baltimore, Md. : 1950) (146:35)||1991|
Characterization of gamma/delta T cell clones isolated from human fetal liver and thymus.
TCR2055 was used in flow cytometry to characterize gamma delta T cells in fetal liver and thymus
|Carding SR,McNamara JG,Pan M,Bottomly K||European journal of immunology (20:1327)||1990|
Intraepithelial T cells of the TcR gamma/delta+ CD8- and V delta 1/J delta 1+ phenotypes are increased in coeliac disease.
TCR2055 was used in immunocytochemistry to investigate the changes of TCR gamma/delta+ CD8- T cells in patients with coeliac disease
|Halstensen TS,Scott H,Brandtzaeg P||Scandinavian journal of immunology (30:665)||1989|