|Tested species reactivity||Human, Mouse|
|Published species reactivity||Human, Mouse, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A synthetic peptide corresponding to 16 amino acids at the amino-terminus of mouse ST2|
|Purification||Antigen affinity chromatography|
|Contains||0.02% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||20 ug/ml|
|Immunofluorescence (IF)||20 ug/ml|
|Immunohistochemistry (IHC)||2 ug/ml|
|Western Blot (WB)||1 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
The immunogenic sequence is common to all three known ST2 isoforms.
A suggested positive control is 293 cell lysate.
PA5-20077 can be used with blocking peptide PEP-0195.
ST2 is a member of a superfamily containing the interleukin-1 receptor and the Toll-like receptors (TLRs). The TLRs are signaling molecules that recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses. ST2 was originally identified as a protein whose production was stimulated by various proliferation-inducing agents such as PDGF and FGF. More recently, it has been shown to negatively regulate IL-1 receptor and Toll-like receptor (TLR) 4 signaling and to maintain endotoxin tolerance. It has been suggested that the inhibition of TLR4 signaling occurs through the association and sequestering of TLR adaptor molecules such as MyD88 and TIRAP.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Deletion of IL-33R attenuates VEGF expression and enhances necrosis in mammary carcinoma.
PA5-20077 was used in immunohistochemistry - paraffin section to study the attenuation of VEGF expression and enhancement of necrosis in mammary carcinoma by deletion of IL-33R
|Milosavljevic MZ,Jovanovic IP,Pejnovic NN,Mitrovic SL,Arsenijevic NN,Simovic Markovic BJ,Lukic ML||Oncotarget (7:18106)||2016|
ST2 deletion increases inflammatory bone destruction in experimentally induced periapical lesions in mice.
PA5-20077 was used in immunohistochemistry - paraffin section to investigate the role of ST2 signaling in the pathogenesis of periapical lesions
|Velickovic M,Pejnovic N,Mitrovic S,Radosavljevic G,Jovanovic I,Kanjevac T,Jovicic N,Lukic A||Journal of endodontics (41:369)||2015|
Expression of interleukin-33 and its receptor ST2 in periapical granulomas and radicular cysts.
PA5-20077 was used in immunohistochemistry - frozen section to determine the expression patterns of IL-33 and ST2 in human periapical lesions
|Velickovic M,Pejnovic N,Petrovic R,Mitrovic S,Jeftic I,Kanjevac T,Lukic A||Journal of oral pathology and medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology (45:70)||2016|