|Tested species reactivity||Bovine|
|Published species reactivity||Bovine|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant bovine IL-4|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|ELISA (ELISA)||See 1 publications below|
PBOIL4I targets IL-4 in ELISA, and WB applications and shows reactivity with Bovine samples.
The PBOIL4I immunogen is recombinant bovine IL-4.
PBOIL4I detects IL-4 which has a predicted molecular weight of approximately 13 kDa.
The protein encoded by this gene is a pleiotropic cytokine produced by activated T cells. This cytokine is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. This gene, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. This gene, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Simultaneous measurement of antigen-stimulated interleukin-1 beta and gamma interferon production enhances test sensitivity for the detection of Mycobacterium bovis infection in cattle.
PBOIL4I was used in ELISA to evaluate the diagnostic markers of Mycobacterium bovis infection in cattle
|Jones GJ,Pirson C,Hewinson RG,Vordermeier HM||Clinical and vaccine immunology : CVI (17:1946)||2010|