|Tested species reactivity||Not Applicable|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||White blood cells.|
|Conjugate||FITC , PE|
|Storage buffer||PBS with 0.2% carrier protein|
|Contains||0.08% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Control (Ctrl)||Assay Dependent|
|Flow Cytometry (Flow)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Flow Cytometry (Flow)||See 1 publications below|
This antibody contains a mix of IgG1 (FITC) and IgG1 (PE) isotypes.
SA1-12320 has been successfully used in FACS procedures to assess non-specific binding of mouse monoclonal antibodies in human samples.
The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody and quote;s species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
EpCAM expression in the prostate cancer makes the difference in the response to growth factors.
SA1-12320 was used in flow cytometry to study the relationship between prostate cancer cell EpCAM expression levels and responsiveness to growth factors
|Yoshida GJ,Saya H||Biochemical and biophysical research communications (443:239)||2014|