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|Tested species reactivity||Bovine, Dog, Camelid, Cat, Goat, Human, Mink, Non-human primate, Sheep, Pig, Rabbit|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||The details of the immunogen for this antibody are not currently available.|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Flow Cytometry (Flow)||1:100 -1:200|
|Functional Assay (FN)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This product is a low-endotoxin, preservative-free formulation. Endotoxin level is <0.01 EU/ug.
For FACS analysis, use 10ul of the suggested working dilution to label 1x10^6 cells in 100ul.
CD14 is a 55 kDa GPI-anchored glycoprotein, constitutively expressed on the surface of mature monocytes, macrophages, and neutrophils, where serves as a multifunctional lipopolysaccharide receptor; it is also released to the serum both as a secreted and enzymatically cleaved GPI-anchored form. CD14 binds lipopolysaccharide molecule in a reaction catalyzed by lipopolysaccharide-binding protein (LBP), an acute phase serum protein. The soluble sCD14 is able to discriminate slight structural differences between lipopolysaccharides and is important for neutralization of serum allochthonous lipopolysaccharides by reconstituted lipoprotein particles. CD14 affects allergic, inflammatory and infectious processes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Elevated Expression of CD160 and 2B4 Defines a Cytolytic HIV-Specific CD8+ T-Cell Population in Elite Controllers.
MA5-16956 was used in flow cytometry to determine if HIV elite controllers have exhausted T cells
|Pombo C,Wherry EJ,Gostick E,Price DA,Betts MR||The Journal of infectious diseases (212:1376)||2015|
Clonal analysis of human dendritic cell progenitor using a stromal cell culture.
MA5-16956 was used in flow cytometry to develop a system to determine human DC development and differentiation
|Lee J,Breton G,Aljoufi A,Zhou YJ,Puhr S,Nussenzweig MC,Liu K||Journal of immunological methods (425:21)||2015|
Diversity, cellular origin and autoreactivity of antibody-secreting cell population expansions in acute systemic lupus erythematosus.
MA5-16956 was used in flow cytometry to examine the antibody repertoire of patients with acute systemic lupus erythematosus.
|Tipton CM,Fucile CF,Darce J,Chida A,Ichikawa T,Gregoretti I,Schieferl S,Hom J,Jenks S,Feldman RJ,Mehr R,Wei C,Lee FE,Cheung WC,Rosenberg AF,Sanz I||Nature immunology (16:755)||2015|
Prolonged proinflammatory cytokine production in monocytes modulated by interleukin 10 after influenza vaccination in older adults.
MA5-16956 was used in flow cytometry to evaluate immune responses by monocyte isolated from young and old adults before and after influenza vaccination
|Mohanty S,Joshi SR,Ueda I,Wilson J,Blevins TP,Siconolfi B,Meng H,Devine L,Raddassi K,Tsang S,Belshe RB,Hafler DA,Kaech SM,Kleinstein SH,Trentalange M,Allore HG,Shaw AC||The Journal of infectious diseases (211:1174)||2015|
NK-cell dysfunction in human renal carcinoma reveals diacylglycerol kinase as key regulator and target for therapeutic intervention.
MA5-16956 was used in flow cytometry to study NK function in solid tumors
|Prinz PU,Mendler AN,Brech D,Masouris I,Oberneder R,Noessner E||International journal of cancer (135:1832)||2014|
Transient decrease in human peripheral blood myeloid dendritic cells following influenza vaccination correlates with induction of serum antibody.
MA5-16956 was used in flow cytometry to examine various DC after flu vaccination.
|Kobie JJ,Treanor JJ,Ritchlin CT||Immunological investigations (43:606)||2014|
Maturation of innate responses to mycobacteria over the first nine months of life.
MA5-16956 was used in flow cytometry to investigate the progression of innate responses to mycobacteria in infants
|Shey MS,Nemes E,Whatney W,de Kock M,Africa H,Barnard C,van Rooyen M,Stone L,Riou C,Kollmann T,Hawn TR,Scriba TJ,Hanekom WA||Journal of immunology (Baltimore, Md. : 1950) (192:4833)||2014|
Evaluation and mechanistic analysis of the cytotoxicity of the acyl glucuronide of nonsteroidal anti-inflammatory drugs.
MA5-16956 was used in flow cytometry to investigate the cytotoxicity of acyl glucuronide.
|Miyashita T,Kimura K,Fukami T,Nakajima M,Yokoi T||Drug metabolism and disposition: the biological fate of chemicals (42:1)||2014|
Capsid-specific T-cell responses to natural infections with adeno-associated viruses in humans differ from those of nonhuman primates.
MA5-16956 was used in flow cytometry to test if rejection of AAV-transduced hepatocytes is due to AAV capsid-specific CD8 positive T cells that become reactivated upon gene transfer.
|Li H,Lasaro MO,Jia B,Lin SW,Haut LH,High KA,Ertl HC||Molecular therapy : the journal of the American Society of Gene Therapy (19:2021)||2011|
Human CD141+ dendritic cells induce CD4+ T cells to produce type 2 cytokines.
MA5-16956 was used in immunocytochemistry to study the role of dendritic cells in relation to T cells
|Yu CI,Becker C,Metang P,Marches F,Wang Y,Toshiyuki H,Banchereau J,Merad M,Palucka AK||Journal of immunology (Baltimore, Md. : 1950) (193:4335)||2014|
CD14; CD14 antigen; Monocyte differentiation antigen CD14; Myeloid cell-specific leucine-rich glycoprotein