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|Tested species reactivity||Bovine|
|Host / Isotype||Yeast|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
For dilution, use a buffer containing carrier protein, e.g. PBS with 1% BSA or a neutral media containing 10% FCS and freeze in aliquots at -70°C.
Activity: The biological activity was determined by bovine neutrophil chemotactic response to bovine IL-8. The ED50 (or 1 unit of activity) for this effect in our laboratories is typically < 15.7% total responding cells at the concentration of Bovine IL-8 at 50 ng/mL.
Expression: The mature form of bovine IL-8 was expressed in Pichia pastoris, X-33. GenBank® Accession # P79255, amino acids 23-101. For protein verification, the expressed protein was N-terminal sequenced and begins with the following 10 amino acids: MSTELRCQCI.
Reconstitute with 0.5 ml of distilled water.
The protein encoded by this gene is a member of the CXC chemokine family. This chemokine is one of the major mediators of the inflammatory response. This chemokine is secreted by several cell types. It functions as a chemoattractant, and is also a potent angiogenic factor. This gene is believed to play a role in the pathogenesis of bronchiolitis, a common respiratory tract disease caused by viral infection. This gene and other ten members of the CXC chemokine gene family form a chemokine gene cluster in a region mapped to chromosome 4q.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bovine Interleukin 8 IL8; C-X-C motif chemokine 8; interleukin-8; neutrophil activating peptide 1
Signal transduction Cell surface receptor mediated signal transduction Cytokine and chemokine mediated signaling pathway Intracellular signaling cascade Calcium mediated signaling NF-kappaB cascade Cell communication Ligand-mediated signaling Immunity and defense T-cell mediated immunity Granulocyte-mediated immunity Macrophage-mediated immunity Developmental processes Mesoderm development Angiogenesis Cell structure and motility Cell motility Cell proliferation and differentiation