Immunohistochemistry analysis of formalin-fixed paraffin-embedded human kidney tissue stained using a GNPDA1 polyclonal antibody (Product # PA5-23236) at 10 ug/ml. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide (101 PENTHILDGNAVDLQAEC 118) of GNPDA1 protein.|
|Storage buffer||PBS with 0.05% BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||10 µg/ml|
|Western Blot (WB)||0.5-1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
GNPDA1 is an allosteric enzyme catalyzing the reversible conversion of D-glucosamine-6-phosphate into D-fructose-6-phosphate and ammonium, a reaction that under physiological conditions proceeds to the formation of fructose-6-phosphate. It is the sole enzyme linking hexosamine systems with glycolytic pathways by transferring hexosamines from structural macromolecules into the glycolytic pathway. GNPDA1 localizes close to the developing acrosome vesicle and in spermatozoa close to the acrosomal region, and may play a role in the acrosome reaction. Human GNPDA1 gene is localized to chromosome 5q21.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.