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Immunohistochemical analysis of LRP-4 was detected in immersion fixed paraffin-embedded sections of human brainstem using Sheep Anti-human/Rat LRP-4 Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47795) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB (brown and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies and processes. This application has not been tested in rat samples.
|Tested species reactivity||Human , Rat|
|Host / Isotype||Sheep / IgG|
|Immunogen||E. coli-derived recombinant human LRP-4 Gly18-Ser344|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 5% trehalose|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||5-15 µg/ml|
|Western Blot (WB)||1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) LRP-1, rhLRP-1B, rhLRP-5, rhLRP-6, and rhLDL R is observed.
Reconstitute at 0.2 mg/mL in sterile PBS.
This gene encodes a member of the low-density lipoprotein receptor-related protein family. The encoded protein may be a regulator of Wnt signaling. Mutations in this gene are associated with Cenani-Lenz syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
LRP-4, low-density lipoprotein receptor-related protein 4, multiple epidermal growth factor-like domains 7, LRP4, low density lipoprotein receptor-related protein 4, KIAA0816, LRP10, MEGF7
SOST2, LRP-4, CLSS, LRP10, Megf7, MEGF7