|Tested species reactivity||Human|
|Host / Isotype||E.coli|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Control (Ctrl)||Assay Dependent|
|ELISA (ELISA)||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
RTNFB20 contains 171 amino acid residues.
Purity: >98% pure by SDS-PAGE and N-terminal sequence analysis.
Activity: The ED50 is determined to be less than 0.05 ng/ml in the cytolysis assay of mouse L929 cells.
Use: TNFb exerts activity in the range of 0.1 to 10 ng/ml for most in vitro applications.
Reconstitution and Dilution: Reconstitute in distilled water to a concentration of 100 ug/ml. After reconstitution, dilute cytokine in a neutral buffer containing carrier protein such as 5-10% FBS or 1-4% high purity BSA (fatty acid free fraction V).
Lyophilized product is stable until expiration if stored desiccated below 0°C. Cytokines diluted as indicated above should be divided into aliquots and stored at -70°C. Recombinant proteins should be stored at concentrations >500 ng/ml to reduce loss of activity.
This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.