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Immunofluorescent analysis of CD106 (green) showing staining in the cytoplasm and membrane of Hela cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a CD106 (Product # PA5-16895) in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant protein encoding aa 26-176 of human CD105|
|Storage buffer||PBS, pH 7.6, with 0.2% BSA|
|Contains||15mM sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:25-1:50|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (IHC)||See 1 publications below|
PA5-16895 targets CD106 in IHC (P) and ICC/IF applications and shows reactivity with Human samples.
The PA5-16895 immunogen is recombinant protein encoding aa 26-176 of human CD105.
CD105 or Endoglin or GP160 is a Type I transmembrane protein, which is highly expressed on human vascular endothelial cells. It exists as an O- and N-glycosylated homodimer. Up-regulation of endoglin expression has been demonstrated in tumor vasculature and proliferating cells, suggesting that it is a proliferation-associated endothelial cell marker. CD105 binds to TGF-beta1 and beta3 with high affinity but not TGF-beta2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Early onset of endothelial cell proliferation in coronary thrombi of patients with an acute myocardial infarction: implications for plaque healing.
PA5-16895 was used in immunohistochemistry to study neovascularization and the timing of endothelial cell proliferation in coronary thrombi following acute myocardial infarction
|Li X,Kramer MC,VAN DER Loos CM,Ploegmakers HJ,DE Boer OJ,Koch KT,Tijssen JG,DE Winter RJ,VAN DER Wal AC||Journal of thrombosis and haemostasis : JTH (10:466)||2012|
CD105 antigen, endoglin, CD105, END, HHT1, ORW, ORW1, ENG
RP11-228B15.2, ORW1, END, HHT1
Signal transduction Cell surface receptor mediated signal transduction Receptor protein serine/threonine kinase signaling pathway Cell communication Cell adhesion-mediated signaling Developmental processes Mesoderm development Heart development Angiogenesis Blood circulation and gas exchange Cell adhesion