|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||E.coli|
|Storage buffer||PBS, pH 7.2|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Control (Ctrl)||Assay Dependent|
|Inhibition Assays (IA)||Cell studies, 8.0-20 µM|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Blocking Assay (BLOCK)||See 1 publications below|
RP-070 was obtained from E. coli transformed with the alpha1-PDX gene. RP-070 can be used in furin inhibition assays. For furin inhibition assays, cells should be treated with 8.0-20 µM alpha1-PDX for 1 hour (see above references). For in vitro assays, 0.1-2.0 µM alpha1-PDX should be used. Purity: >80% (most lots are 80-95%) NOTE: Freezing this product may result in damage of the protein. NOTE: Once rehydrated, this protein retains optimal reactivity for 2-4 days at 4 degrees C. Longer storage time can be achieved by storing in a serum rich medium. RP-070 can be used for protein biochemical, intracellular trafficking, and protein kinetic assays.
Molecular weight is approximately 46.7 kDa.
Alpha1-PDX is a potent and selective inhibitor of furin (Ki = 0.6 nM). Alpha1-PDX was engineered by the construction of a variant of alpha1-antitrypsin that contains a minimal furin consensus sequence, RXXR. Cellular expression of alpha1-PDX effectively blocks the furin-dependent processing of protein precursors. In addition, extracellular application of alpha1-PDX can also block the processing of furin substrates. Alpha1-PDX has been shown to inhibit the processing of biologically significant proteins including many viral envelope glycoproteins, bacterial toxins, receptors and growth factors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
alpha1-Antitrypsin Portland, a bioengineered serpin highly selective for furin: application as an antipathogenic agent.
RP-070 was used in blocking/activating experiment to investigate the function and potential utility of a bioengineered serpin alpha1-Antitrypsin Portland
|Jean F,Stella K,Thomas L,Liu G,Xiang Y,Reason AJ,Thomas G||Proceedings of the National Academy of Sciences of the United States of America (95:7293)||1998|