|Tested species reactivity||Mouse|
|Published species reactivity||Not Applicable|
|Host / Isotype||Armenian hamster / IgG|
|Storage buffer||PBS with 4-5mg/ml BSA|
|Contains||0.02% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||0.2 ug/10^6 cells|
|Immunohistochemistry (Frozen) (IHC (F))||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Blocking Assay (BLOCK)||See 1 publications below|
MA5-17657 targets CD3e/TCRE in FACS, IHC (F), and WB applications and shows reactivity with Mouse samples.
The MA5-17657 immunogen is h-2Kb sp.
T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||5 µg/ml||
Hair loss and defective T- and B-cell function in mice lacking ORAI1.
MA5-17657 was used in blocking or activating experiment to characterize the physiological consequences of ORAI1 deficiency
|Gwack Y,Srikanth S,Oh-Hora M,Hogan PG,Lamperti ED,Yamashita M,Gelinas C,Neems DS,Sasaki Y,Feske S,Prakriya M,Rajewsky K,Rao A||Molecular and cellular biology (28:5209)||2008|