|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||human peripheral blood T lymphocytes|
|Storage buffer||PBS with 0.2% carrier protein|
|Contains||0.08% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
FACS procedure using PBMCs: Add 10µl of MA1-12030/10^6 PBMC in 100µl PBS. Mix gently and incubate for 15 minutes on ice. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze.
FACS procedure using whole blood: Add 10µl of MA1-12030/100µl of whole blood. Mix gently and incubate for 15 minutes at room temperature. Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze.
CD8 molecule is composed of two chains termed alpha and beta. CD8 is found on a T cell subset of normal cytotoxic / suppressor cells which make up approximately 20 to 35 % of human peripheral blood lymphocytes. The CD8 antigen is also detected on natural killer cells, 80% of thymocytes, on a subpopulation of 30% of peripheral blood null cells and 15 to 30% of bone marrow cells.
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