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Western blot analysis from lysates of human cartilage tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-human CRTAC1 Isoform 1 Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47710) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody. A specific band was detected for CRTAC1 at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions.
|Tested species reactivity||Human|
|Host / Isotype||Sheep / IgG|
|Immunogen||Mouse myeloma cell line NS0-derived recombinant human CRTAC1 Isoform 1 Ser28-Cys661|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 5% trehalose|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1-10 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody detects human CRTAC1 Isoform 1 in direct ELISAs and Western blots. In Western blots, approximately 10% cross-reactivity with recombinant human CRTAC1 Isoform 2 is observed.
Reconstitute at 0.2 mg/mL in sterile PBS.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
acidic secreted protein in cartilage; ASPIC1; ASPIC1CEP-68acidic secreted protein in cartilage; cartilage acidic protein 1; CEP68; chondrocyte expressed protein 68 kDa CEP-68; FLJ10320
ASPIC; ASPIC1; CEP-68; CEP68; CRTAC1