|Control (Ctrl)||Assay Dependent|
|Gel Shift (GS)||Assay dependent|
|Western Blot (WB)||Assay dependent|
|Gel Shift (GS)||See 3 publications below|
|Tested Species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Insect|
|Storage conditions||-80° C, Avoid Freeze/Thaw Cycles|
RP-500A contains 50 ul of recombinant human glucocorticoid receptor at 3.5 mg/ml. RP-500B contains 20 ul of oligonucleotide at 100 ng/ul.
Each RP-500 is sufficient for 50 gel shift reactions or 50 lanes for Western blot detection with any ABR polyclonal Anti-human Glucocorticoid Receptor Antibody (PA1-510A, PA1-511A, PA1-512). Gel shift and Western blot protocols are supplied with RP-500 or available upon request. The components of this kit may be used to perform gel shift assays and with PA1-510A, Anti-Glucocorticoid Receptor antibody, gel super shift assays. This complete system can be used as a control system into which the researcher may introduce other components such as other putative response elements or other glucocorticoid receptors (mutants, from various tissue sources, etc.). The recombinant protein may also be used to perform DNase I footprinting experiments, as a control in Western blot experiments or for in vitro transcription assays.
RP-500A is recombinant human glucocorticoid receptor (full length) that was expressed in an insect cell expression system and enriched by ammonium sulfate precipitation followed by dialysis in KED buffer with 20% glycerol.
RP-500B is an oligonucleotide double stranded DNA representing the human tyrosine amino transferase gene response element with the following sequences: 5 and quote;...CTA GGC TGT ACA GGA TGT TCT GCC TAG...3 and quote;
3 and quote;...GAT CCG ACA TGT CCT ACA AGA CGG ATC...5 and quote;
Steroid receptors are ligand-dependent, intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate hormone. Glucocorticoids are a family of steroids necessary for the regulation of energy metabolism, and the immune and inflammatory responses. These compounds exert their effect through their interaction with the glucocorticoid receptor (GR) and that complex's subsequent association with DNA. All normal mammalian tissues examined to date have been shown to contain glucocorticoid receptor (GR).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: glucocorticoid nuclear receptor variant 1; Glucocorticoid receptor; GR; GR Kit; Nuclear receptor subfamily 3 group C member 1; nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor)
Gene Aliases: GCCR; GCR; GCRST; GR; GRL; NR3C1
UniProt ID: (Human) P04150
Entrez Gene ID: (Human) 2908
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