|ELISA (ELISA)||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
|Tested Species reactivity||Mouse|
|Host / Isotype||E.coli|
|Storage buffer||PBS with 0.1% BSA|
|Storage conditions||-80° C, Avoid Freeze/Thaw Cycles|
Assay Used to Measure Bioactivity: Interferon was titrated with the use of the cytopathic effect inhibition assay as described [Familletti, et al. (1981), Methods in Enzymology, Vol. 78 (S. Pestka, ed.), Academic Press, New York, 387-394] with the exception that EMCV rather than VSV was used as the challenge. The activity was determined relative to a lab standard of Mu IFN-alpha-A which was calibrated to the NIH mouse IFN-alpha- standard (Ga02-901-511). Mouse (L929/EMCV) in this assay the EC50 for IFN is ~ 5U/ml. Lot Activity was derived from multiple determinations in the above assay.
Store at -70°C or below for retention of full activity. Thaw product vial by incubation in cold tap water until just thawed. Aliquot and refreeze unused contents to minimize effects of freeze thawing. Refreezing should be done on dry ice or in a dry ice/alcohol bath. Further dilution of product should be in buffers containing protein such as 0.1% BSA or tissue culture media with serum. Dilution for freezing of material below 2x10^5 units/ml is not recommended. One freeze thaw cycle is equivalent to thawing an aliquot prepared from the material received.
The protein encoded by this gene is produced by macrophages and has antiviral activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: IFN-alpha-1; IFNA; interferon alpha family gene 1; Interferon alpha-1; Murine interferon Alpha
Gene Aliases: Ifa1; Ifna1
UniProt ID: (Mouse) P01572
Entrez Gene ID: (Mouse) 15962
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