|Control (Ctrl)||Assay Dependent|
|Tested Species reactivity||Yeast|
|Host / Isotype||E.coli|
|Storage buffer||0.02M HEPES, pH 7.0, with 10% glycerol|
|Storage conditions||-80° C, Avoid Freeze/Thaw Cycles|
This protein corresponds to yeast Thioredoxin 2 (1-104 aa), which has a molecular weight of 11.3 kDa.
Specific activity: 9.7 U/mg, where one unit will cause a delta-A650 of 1 in 1 min at 25°C in the insulin reduction assay.
Purity: >95% by SDS-PAGE.
Thioredoxins (Trx) are small, multi-functional proteins with oxidoreductase activity and are ubiquitous in essentially all living cells. Trx contains a redox-active disulfide/dithiol group within the conserved Cys-Gly-Pro-Cys active site. The two cysteine residues in the conserved active centers can be oxidized to form intramolecular disulfide bonds. Reduction of the active site disulfide in oxidized Trx is catalyzed by Trx reductase with NADPH as the electron donor. The reduced Trx is a hydrogen donor for ribonucleotide reductase, the essential enzyme for DNA synthesis, and a potent general protein disulfide reductase with numerous functions in growth and redox regulations. Specific protein disulfide targets for reduction by Trx include protein disulfide isomerase (PDI) and a number of transcription factors such as p53 , NF-kB and AP-1 (T1-151). Trx is also capable of removing H2O2, particularly when it is coupled with either methionine sulfoxide reductase or several isoforms of peroxiredoxins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Thioredoxin II; Thioredoxin-1; Thioredoxin-2; TR-II; Trx2p
Gene Aliases: G7746; LMA1; TRX1; TRX2; YGR209C
UniProt ID: (Baker's yeast) P22803
Entrez Gene ID: (Baker's yeast) 853123
Molecular Function: oxidoreductase
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