|ELISA (ELISA)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
|Tested Species reactivity||Mammal|
|Host / Isotype||Mouse|
|Immunogen||Synthetic GLP-1(9-37) adsorbed onto aluminum hydroxide gel.|
|Storage buffer||PBS, pH 7.4|
|Contains||15mM sodium azide|
|Storage conditions||4° C, store in dark|
Specificity: ABS 046-03-02 binds GLP-1 (1-37), GLP-1 (7-37) and GLP-1 (9-37). This antibody shows 0-16% cross-Specificity with C-terminally amidated GLP-1 in various circumstances.
Reactivity: ABS 046-03-02 binds free GLP-1 (1-37), GLP-1 (7-37) and GLP-1 (9-37) in solution. Two different ELISA sandwich setups can be used for measuring non-amidated GLP-1 forms: The first uses ABS 046-03 as a capture antibody with biotinylated detection antibody ABS 033-10-02. This shows ~5% cross-reactivity with C-terminally amidated GLP-1. The second combination uses HYB 147-12 as a capture antibody with biotinylated detection antibody ABS 046-03-02, resulting in ~16% cross-reactivity with C-terminally amidated GLP-1. Results show detection limits of 44 pmol/L which is 10-20 times higher than the basal concentration of GLP-1, so the assays have to be optimized.
NOTE: Concentration is lot-dependent and can vary from 0.85-1.15 mg/ml
GLP-1(7-36) amide is the principal active form of GLP-1, the other being GLP-1(7-37). GLP-1 is a peptide hormone of the glucagon family, produced by the L cells of the intestinal mucosa from the same prohormone as glucagon. The active forms are potent stimulators of glucose-dependent insulin secretion. The sequence of GLP-1 is fully conserved in all mammalian species examined so far.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: GLP-1 receptor; GLP-1-R; GLP-1R; GLP1 receptor; GLP1, GLP-1, GLP2, GLP-2, GRPP, glicentin-related polypeptide; glucagon-like peptide 1; Glucagon-like peptide 1 receptor; glucagon-like peptide 2, Glicentin, GCG; glucagon-like peptide-1 receptor; pancreatic beta cell receptor for the gluco-incretin hormone glucagon-like peptide 1; seven transmembrane helix receptor
Gene Aliases: Glip; GLP-1; GLP-1-R; GLP-1R; GLP1R; GLP1Rc; Glpr; RATGL1RCP
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.