|Tested species reactivity||Rat|
|Published species reactivity||Rat|
|Host / Isotype||Mouse / Cocktail|
|Immunogen||Rat thymocyte membrane glycoproteins|
|Conjugate||FITC , PE|
|Storage buffer||PBS with 1% BSA, 5% sucrose|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C, store in dark, DO NOT FREEZE!|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Neat|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Miscellaneous PubMed (MISC)||See 4 publications below|
MA5-16727 contains a monoclonal mixture of FITC-conjugated CD3 (clone #IF4, Mouse IgM) and RPE-conjugated CD8 (clone #OX-8, Mouse IgG1). This antibody is a dual-color reagent recognizing rat CD3 and CD8 cell surface antigens. Clone IF4 recognizes rat CD3, a cell surface antigen expressed by mature lymphocytes. Clone OX8 recognizes rat CD8, a cell surface glycoprotein expressed by thymocytes and by the suppressor/cytotoxic subset of peripheral blood T cells. CD8 expression has also been reported upon rat macrophages.
Reconstitute with 1 ml of distilled water. After reconstitution, store product undiluted at 4°C in the dark.
For FACS analysis, use 10ul of the suggested working dilution to label 1x10^6 cells in 100ul.
putative component of the CD3 T-cell receptor.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Cyclosporine facilitates chimeric and inhibits nonchimeric tolerance after posttransplant total lymphoid irradiation.
MA5-16727 was used in flow cytometry to discuss tolerance to ACI heart allografts using rats.
|Lan F,Hayamizu K,Strober S||Transplantation (69:649)||2000|
Comparison of chimeric acid and non-chimeric tolerance using posttransplant total lymphoid irradiation: cytokine expression and chronic rejection.
MA5-16727 was used in flow cytometry to study tolerance induction using heart allografts in Lewis rat.
|Hayamizu K,Lan F,Huie P,Sibley RK,Strober S||Transplantation (68:1036)||1999|
Monocyte-derived dendritic cell precursors facilitate tolerance to heart allografts after total lymphoid irradiation.
MA5-16727 was used in flow cytometry to discuss factors that promote tolerance in a rat ACI heart allografts model.
|Hayamizu K,Huie P,Sibley RK,Strober S||Transplantation (66:1285)||1998|
Donor blood monocytes but not T or B cells facilitate long-term allograft survival after total lymphoid irradiation.
MA5-16727 was used in flow cytometry to determine the subset(s) of cells in donor blood that promote long-term allograft survival in rats.
|Hayamizu K,Zeng D,Huie P,Garcia-Ojeda ME,Bloch DA,Fong L,Engleman EG,Sibley RK,Strober S||Transplantation (66:585)||1998|