|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A 16 amino acid peptide from near the center of human Integrin alpha 4.|
|Purification||Antigen affinity chromatography|
|Contains||0.02% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||2 ug/ml|
|Immunofluorescence (IF)||2 µg/ml|
|Western Blot (WB)||1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunocytochemistry (ICC)||See 1 publications below|
Despite its predicted molecular weight, Integrin alpha4 often migrates at a higher molecular weight in SDS-PAGE.
PA5-20599 can be used with blocking peptide PEP-0719.
The integrin alpha 4 (also known as CD49d and ITGA4) belongs to the integrin alpha chain family of proteins. Integrins are heterodimeric integral membrane proteins composed of an alpha and beta chains. Alpha4 chain associates with either beta1 or beta7 chain. It has been demonstrated that the putative ligand-binding sites of both integrin alpha4beta1 and alpha4beta7 is located on the alpha4 chain. These ligands included Madcam, VCAM, and fibronectin. Madcam is known as the principal ligand for integrin alpha4beta7. Recently it was also demonstrated that HIV-1 envelope can mimic Madcam by binding to and signaling though integrin alpha4beta1, the gut mucosal homing receptor for peripheral T cells. At least two isoforms are known to exist.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Specific phosphorylations transmit signals from leukocyte ß2 to ß1 integrins and regulate adhesion.
PA5-20599 was used in immunocytochemistry to study leukocyte function-associated antigen 1 signaling with a focus on integrins
|Uotila LM,Jahan F,Soto Hinojosa L,Melandri E,Grönholm M,Gahmberg CG||The Journal of biological chemistry (289:32230)||2014|