Immunohistochemical analysis of paraffin-embedded human breast ductal myoepithelium (A) and lymph tissue (B), showing cytoplasmic (A) and membrane (B) localization using CD10/MME monoclonal antibody (Product # MA5-15231) followed with DAB staining (A) and AEC staining (B).
|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||Purified recombinant fragment of human CD-10 expressed in E. Coli.|
|Contains||0.03% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (IHC)||1/200 - 1/1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-15231 targets CD10 in IHC applications and shows reactivity with Human samples.
The MA5-15231 immunogen is purified recombinant fragment of human CD-10 expressed in E. Coli.
CD10 is a 100kDa glycoprotein, also designated Common Acute Lymphocytic Leukemia Antigen (CALLA). It is a cell surface enzyme with neutral metalloendopeptidase activity which inactivates a variety of biologically active peptides. CD10 is expressed on the cells of lymphoblastic, Burkitt and quote;s, and follicular germinal center lymphomas, and on cells from patients with chronic myelocytic leukemia (CML). It is also expressed on the surface of normal early lymphoid progenitor cells, immature B cells within adult bone marrow and germinal center B cells within lymphoid tissue. CD10 is also present on breast myoepithelial cells, bile canaliculi, fibroblasts, with especially high expression on the brush border of kidney and gut epithelial cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.