|Tested species reactivity||Human, Mouse|
|Published species reactivity||Non-human primate, Human, Mouse, Chicken, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human PKR that contains threonine 451.|
|Purification||Antigen affinity chromatography|
|Storage buffer||Dulbecco's PBS, pH 7.3, with 1mg/ml BSA, 50% glycerol|
|Contains||0.05% sodium azide|
|Tested Applications||Dilution *|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 5 publications below|
PRKR, a 68KDa protein, is an IFN-induced serine/threonine protein kinase consisting of two N-terminal RNA-binding regulatory domains and a functional C-terminal serine/threonine protein kinase domain. Activated by dsRNA produced during viral infections, it plays a key role in IFN induced-innate antiviral response, virus-induced apoptosis, cell growth and differentiation. Upon activation, PRKR gets autophosphorylated and catalyzes the phosphorylation of elF2 alpha subunit, which leads to an inhibition of protein synthesis. It induces apoptosis by up-regulating Fas expression and mediates FADD/Caspase-8 death signaling pathway. Upon viral infection, it functions as a dual protein, sequentially activating both cell survival and cell death pathways using kinase independent and dependent strategies. PRKR regulates multiple pathways which include NF-kappaB activation, p53, p38, and PDGF signaling pathway. Its role has been implicated in tumor suppression and malignancy.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
The small molecule '1-(4-biphenylylcarbonyl)-4-(5-bromo-2-methoxybenzyl) piperazine oxalate' and its derivatives regulate global protein synthesis by inactivating eukaryotic translation initiation factor 2-alpha.
44-668G was used in western blot to determine regulation of global protein synthesis by inactivating eukaryotic translation initiation factor 2-alpha by the small molecule '1-(4-biphenylylcarbonyl)-4-(5-bromo-2-methoxybenzyl) piperazine oxalate' and its
|Hong MN,Nam KY,Kim KK,Kim SY,Kim I||Cell stress and chaperones (21:485)||2016|
|The infectious bursal disease virus RNA-binding VP3 polypeptide inhibits PKR-mediated apoptosis.||Busnadiego I,Maestre AM,Rodríguez D,Rodríguez JF||PloS one (7:null)||2012|
|Human||Not Cited||Transcriptional gene silencing of HIV-1 through promoter targeted RNA is highly specific.||Suzuki K,Ishida T,Yamagishi M,Ahlenstiel C,Swaminathan S,Marks K,Murray D,McCartney EM,Beard MR,Alexander M,Purcell DF,Cooper DA,Watanabe T,Kelleher AD||RNA biology (8:1035)||2011|
|Human||Not Cited||Inhibition of double-stranded RNA-dependent protein kinase strongly decreases cytokine production and release in peripheral blood mononuclear cells from patients with Alzheimer's disease.||Couturier J,Page G,Morel M,Gontier C,Claude J,Pontcharraud R,Fauconneau B,Paccalin M||Journal of Alzheimer's disease : JAD (21:1217)||2011|
|Mouse||Not Cited||The double-stranded RNA-activated protein kinase mediates radiation resistance in mouse embryo fibroblasts through nuclear factor kappaB and Akt activation.||von Holzen U,Pataer A,Raju U,Bocangel D,Vorburger SA,Liu Y,Lu X,Roth JA,Aggarwal BB,Barber GN,Keyomarsi K,Hunt KK,Swisher SG||Clinical cancer research : an official journal of the American Association for Cancer Research (13:6032)||2007|