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|Tested species reactivity||Human, Mouse|
|Published species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human PKR that contains threonine 451.|
|Purification||Antigen affinity chromatography|
|Storage buffer||Dulbecco's PBS, pH 7.3, with 1mg/ml BSA, 50% glycerol|
|Contains||0.05% sodium azide|
|Tested Applications||Dilution *|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 3 publications below|
PRKR, a 68KDa protein, is an IFN-induced serine/threonine protein kinase consisting of two N-terminal RNA-binding regulatory domains and a functional C-terminal serine/threonine protein kinase domain. Activated by dsRNA produced during viral infections, it plays a key role in IFN induced-innate antiviral response, virus-induced apoptosis, cell growth & differentiation. Upon activation, PRKR gets autophosphorylated and catalyzes the phosphorylation of elF2 alpha subunit, which leads to an inhibition of protein synthesis. It induces apoptosis by up-regulating Fas expression and mediates FADD/Caspase-8 death signaling pathway. Upon viral infection, it functions as a dual protein, sequentially activating both cell survival & cell death pathways using kinase independent and dependent strategies. PRKR regulates multiple pathways which include NF-kappaB activation, p53, p38, & PDGF signaling pathway. Its role has been implicated in tumor suppression & malignancy.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Human||Not Cited||Transcriptional gene silencing of HIV-1 through promoter targeted RNA is highly specific.||Suzuki K,Ishida T,Yamagishi M,Ahlenstiel C,Swaminathan S,Marks K,Murray D,McCartney EM,Beard MR,Alexander M,Purcell DF,Cooper DA,Watanabe T,Kelleher AD||RNA biology (8:1035)||2011|
|Human||Not Cited||Inhibition of double-stranded RNA-dependent protein kinase strongly decreases cytokine production and release in peripheral blood mononuclear cells from patients with Alzheimer's disease.||Couturier J,Page G,Morel M,Gontier C,Claude J,Pontcharraud R,Fauconneau B,Paccalin M||Journal of Alzheimer's disease : JAD (21:1217)||2011|
|Mouse||Not Cited||The double-stranded RNA-activated protein kinase mediates radiation resistance in mouse embryo fibroblasts through nuclear factor kappaB and Akt activation.||von Holzen U,Pataer A,Raju U,Bocangel D,Vorburger SA,Liu Y,Lu X,Roth JA,Aggarwal BB,Barber GN,Keyomarsi K,Hunt KK,Swisher SG||Clinical cancer research : an official journal of the American Association for Cancer Research (13:6032)||2007|
double stranded RNA activated protein kinase; dsRNA-activated kinase; EC 22.214.171.124; eIF-2 alpha; eIF-2A protein kinase 2; eukaryotic translation initiation factor 2 alpha kinase 2; Eukaryotic translation initiation factor 2-alpha kinase 2; IFN- type I-induced and dsRNA-activated kinase; IFN-induced and double-stranded RNA-activated kinase; Interferon-induced, double-stranded RNA-activated protein kinase; interferon-inducible elF2alpha kinase; Interferon-inducible RNA-dependent protein kinase; P1/eIF-2A protein kinase; p68 kinase; PKR; protein kinase R; Protein kinase RNA-activated; protein kinase, interferon inducible double stranded RNA dependent; protein kinase, interferon-inducible double stranded RNA dependent; protein phosphatase 1, regulatory subunit 83; Serine/threonine-protein kinase TIK; T-cell viral integration site; Tyrosine-protein kinase EIF2AK2
2310047A08Rik; 4732414G15Rik; AI467567; AI747578; EIF2AK1; EIF2AK2; PKR; PPP1R83; PRKR; Tik