|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunohistochemistry (Paraffin) (IHC (P))||2-4 µg/ml|
|Immunoprecipitation (IP)||2 µg/ml|
|Western Blot (WB)||1 µg/mL|
|Western Blot (WB)||See 4 publications below|
|Immunohistochemistry (IHC)||See 10 publications below|
|Miscellaneous PubMed (MISC)||See 1 publications below|
|Tested Species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Recombinant human 1433 sigma protein|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage conditions||4° C|
MA5-11663 targets 14-3-3 sigma in ICC/IF, IHC (P), IP, and WB applications and shows reactivity with Human samples.
The MA5-11663 immunogen is recombinant human 1433 sigma protein.
14.3.3 proteins are a family of small, widely expressed, highly conserved cytosolic proteins. 14.3.3 proteins bind to and influence the activities of a diverse group of molecules involved in signal transduction, cell cycle regulation and apoptosis, including Raf, PKC, Bad, Cbl, and c-Bcr. Interactions between 14.3.3 and target proteins are strongly influenced by the phosphorylation state of 14.3.3 and the target protein. 14.3.3 sigma shares cyclin-Cdk2 binding motifs with different cell cycle regulators, including p107, p130, p21Cip1, p27Kip1, and p57Kip2 and is associated with cyclin-Cdk complexes in vitro and in vivo.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 14-3-3 protein sigma; 14-3-3 sigma; Epithelial cell marker protein 1; Stratifin
Gene Aliases: HME1; SFN; YWHAS
UniProt ID: (Human) P31947
Entrez Gene ID: (Human) 2810
Molecular Function: chaperone
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