|ELISA (ELISA)||0.1 to 0.4 µg/ml|
|Flow Cytometry (Flow)||5-10 µl/10^6 cells|
|Immunocytochemistry (ICC)||1-4 µg/mL|
|Immunofluorescence (IF)||1-4 µg/mL|
|Immunohistochemistry (Frozen) (IHC (F))||1-4 µg/mL|
|Immunoprecipitation (IP)||4 ug/mL|
|Western Blot (WB)||1-4 µg/mL|
|Tested Species reactivity||Tag|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Recombinant (His) 6-p53 protein.|
|Storage buffer||PBS, pH 7.4|
|Storage conditions||4° C, do not freeze|
MA1-4806 detects any kind of 6X-His tagged proteins in cells and complex cellular lysates.
MA1-4806 has been successfully used in flow cytometry, immunohistochemistry (frozen), immunofluorescence, Western blot, immunoprecipitation, and ELISA procedures. By Western blot this antibody detects recombinant proteins containing an epitope of at least 6 histidine residues, located at the N-terminus, C-terminus or at both termini. A higher number of histidine residues leads to an increased binding affinity of the antibody.
The MA1-4806 immunogen is recombinant (His) 6-p53 protein
The His tag is a synthetic oligo peptide consisting of 6 consecutive histidine residues (HHHHHH). It is commonly expressed as a tag at either N-or C-terminal regions of recombinant proteins to allow isolation or purification by immobilized metal affinity chromatography. The affinity of the histidine-tag motif to Ni2+ by chelation is strong and selective enough to enable purification of the protein to homogeneity by affinity chromatography on a Ni2+-NTA adsorbent. His tag-specific antibodies are used to facilitate detection or coimmunopreciptation of tagged proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 6His tag; 6X His; 6X His eptope tag; His Tag; Histidine Epitope Tag; Histidine Tag
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