|Western Blot (WB)||1-3 ug/ml|
|Tested Species reactivity||Tag|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic construct corresponding to amino acids HHHHHH|
|Contains||0.09% sodium azide|
|Storage conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
ABfinity™ recombinant antibodies are rabbit monoclonal antibodies, unmatched for producing superior results. ABfinity™ antibodies are developed by immunizing animals, screening for functionality, cloning the immunogen-specific antibody genes into high-level mammalian expression vectors, produced on a large scale and purified with Protein A.
ABfinity™ oligoclonal antibodies comprise a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds—the sensitivity of a polyclonal antibody with the specificity of a monoclonal, all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody—recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets when compared with monoclonal antibodies—an oligoclonal antibody has a known mixture of light and heavy chains. This exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
The His tag is a synthetic oligo peptide consisting of 6 consecutive histidine residues (HHHHHH). It is commonly expressed as a tag at either N-or C-terminal regions of recombinant proteins to allow isolation or purification by immobilized metal affinity chromatography. The His tag specific antibody is used to facilitate detection or coimmunopreciptation of tagged proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 6His tag; 6X His; 6X His eptope tag; His Tag; Histidine Epitope Tag; Histidine Tag
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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