|Flow Cytometry (Flow)||1/10|
|Immunofluorescence (IF)||Assay dependent|
|Tested Species reactivity||Tag|
|Host / Isotype||Mouse / IgG1|
|Immunogen||PAX6 transcription factor linked to histidine tag.|
|Storage buffer||PBS, pH 7.4, with 1% BSA|
|Contains||0.09% sodium azide|
|Storage conditions||4°C or -20°C if preferred, store in dark|
MA1-81891 recognizes proteins containing the motiff H-H-H-H-H-H and is therefore of value in detecting proteins containing histidine tags. The antibody may be used to track the purification of proteins expressed in bacteria, yeast or insect cells. It reacts aginst all His-tagged proteins tested so far.
For FACS analysis, use 10ul of the suggested working dilution to label 1x10^6 cells in 100ul.
Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. Expression of recombinant proteins in E. coli as a fusion protein with neighboring histidine residues is one of the most popular methods of epitope tags. The affinity of the histidine-tag motif to Ni2+ by chelation is strong and selective enough to enable purification of the protein to homogeneity by affinity chromatography on a Ni2+-NTA adsorbent.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 6His tag; 6X His; 6X His eptope tag; His Tag; Histidine Epitope Tag; Histidine Tag
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.