|Western Blot (WB)||1:1000-1:5000|
|Tested Species reactivity||Tag|
|Host / Isotype||Goat / IgG|
|Immunogen||6-His (HHHHHH) conjugated to KLH|
|Purification||Antigen affinity chromatography|
|Storage buffer||50mM HEPES buffered saline, pH 7.1, with 0.2% BSA, 0.1M NaCl|
|Storage conditions||4° C, do not freeze|
This antibody does not detect all N-terminal 6-His tagged proteins.
Antibody concentration was determined by extinction coefficient prior to conjugation: absorbance at
280 nm of 1.4 equals 1.0 mg of IgG. Molar enzyme/antibody protein ratio is 4:1.
The His tag is a synthetic oligo peptide consisting of 6 consecutive histidine residues (HHHHHH). It is commonly expressed as a tag at either N-or C-terminal regions of recombinant proteins to allow isolation or purification by immobilized metal affinity chromatography. The affinity of the histidine-tag motif to Ni2+ by chelation is strong and selective enough to enable purification of the protein to homogeneity by affinity chromatography on a Ni2+-NTA adsorbent. His tag-specific antibodies are used to facilitate detection or coimmunopreciptation of tagged proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 6His tag; 6X His; 6X His eptope tag; His Tag; Histidine Epitope Tag; Histidine Tag
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