|Western Blot (WB)||1:1,000-1:5,000|
|Tested Species reactivity||Tag|
|Host / Isotype||Chicken / IgY|
|Immunogen||His tagged synthetic peptide.|
|Contains||0.02% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
PA1-9531 has been successfully used in Western blot, ELISA and immunocytochemistry procedures. By Western blot, PA1-9531 detects a His-Tag fusion protein protein representing His Tag.
PA1-9531 immunogen is a His tagged synthetic peptide.
The His tag is a synthetic oligo peptide consisting of 6 consecutive histidine residues (HHHHHH). It is commonly expressed as a tag at either N-or C-terminal regions of recombinant proteins to allow isolation or purification by immobilized metal affinity chromatography. The affinity of the histidine-tag motif to Ni2+ by chelation is strong and selective enough to enable purification of the protein to homogeneity by affinity chromatography on a Ni2+-NTA adsorbent. His tag-specific antibodies are used to facilitate detection or coimmunopreciptation of tagged proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 6His tag; 6X His; 6X His eptope tag; His Tag; Histidine Epitope Tag; Histidine Tag
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