|Western Blot (WB)||1:250|
|Western Blot (WB)||See 2 publications below|
|Miscellaneous PubMed (MISC)||See 2 publications below|
|Tested Species reactivity||Tag|
|Published species reactivity||Yeast|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues H H H H H H.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
PA1-983B detects 6x-His Epitope tagged proteins
PA1-983B has been successfully used in Western blot procedures.
The PA1-983B immunogen is a synthetic peptide corresponding to residues H H H H H H.
The His tag is a synthetic oligo peptide consisting of 6 consecutive histidine residues (HHHHHH). It is commonly expressed as a tag at either N-or C-terminal regions of recombinant proteins to allow isolation or purification by immobilized metal affinity chromatography. The affinity of the histidine-tag motif to Ni2+ by chelation is strong and selective enough to enable purification of the protein to homogeneity by affinity chromatography on a Ni2+-NTA adsorbent. His tag-specific antibodies are used to facilitate detection or coimmunopreciptation of tagged proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 6His tag; 6X His; 6X His eptope tag; His Tag; Histidine Epitope Tag; Histidine Tag
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