|Flow Cytometry (Flow)||1 µg/10^6 cells|
|Immunohistochemistry (Paraffin) (IHC (P))||1:400|
|Western Blot (WB)||1:2000|
|Tested Species reactivity||Human, Mouse, Rabbit, Rat|
|Host / Isotype||Mouse / IgG2b, kappa|
|Immunogen||50 kDa N-terminal extracellular loop of ABCA1|
|Contains||0.05% sodium azide|
|Storage conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
Mutations in the ABCA1 gene (ATP-binding cassette transporter 1) have been reported in Tangier disease (TD). TD is an autosomal recessive disorder characterized by an absence of plasma HDL, cholesterol ester depositing in the reticulo-endothelial system and deviations in cellular lipid trafficking. ABCA1 mediates the apo-A1 associated export of cholesterol and phospholipids from the cell. It is expressed on the plasma membrane and the Golgi complex, and is regulated by cholesterol flux. Regulation of the cholesterol flux between HDL and macrophages is competitive between ABCA1 and SR-BI.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: ABC-1; ABC1; ATP-binding cassette 1; ATP-binding cassette sub-family A member 1; ATP-binding cassette transporter 1; ATP-binding cassette transporter A1; ATP-binding cassette, sub-family A (ABC1), member 1; CERP; Cholesterol efflux regulatory protein; HDLDT1; membrane-bound; TGD
Gene Aliases: ABC-1; ABC1; ABCA1; CERP; HDLDT1; TGD
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