|Flow Cytometry (Flow)||Assay dependent.|
|Immunocytochemistry (ICC)||1:20 - 1:50|
|Immunohistochemistry (Frozen) (IHC (F))||1:20|
|Immunohistochemistry (Paraffin) (IHC (P))||1:20|
|Western Blot (WB)||1:20 - 1:50|
|Tested Species reactivity||Human|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||fusion protein of bacterial MBP and rat Mrp2, containing the 202-amino acid COOH terminal end|
|Storage buffer||tissue culture supernatant with 0.7% BSA|
|Contains||0.1% sodium azide|
|Storage conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
For immunohistochemistry (paraffin) applications, it is recommended to pretreat antibody with 0.01 M citric acid, pH 6.0, in distilled water at 100°C 3 times for 5 minutes.
M2III-5 reacts with an internal epitope of MRP2, a 190-200 kD transmembrane protein earlier known as the canalicular multi-organic anion transporter cMOAT, absent in patients with the Dubin-Johnson syndrome, an autosomal recessive liver disorder characterized by chronic conjugated hyperbilirubinemia. MRP2 is a member of the MRP family of multidrug resistance related proteins, and MRP2 overexpression has been observed in a subset of cisplatin resistant cell lines.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: ATP-binding cassette sub-family C member 2; ATP-binding cassette, sub-family C (CFTR/MRP), member 2; Canalicular multidrug resistance protein; Canalicular multispecific organic anion transporter 1; Human MRP2; Multidrug resistance-associated protein 2
Gene Aliases: ABC30; ABCC2; CMOAT; CMOAT1; CMRP; DJS; MRP2
UniProt ID: (Human) Q92887
Entrez Gene ID: (Human) 1244
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