|Immunocytochemistry (ICC)||10 µg/ml|
|Immunofluorescence (IF)||20 ug/ml|
|Western Blot (WB)||0.5-1 ug/ml|
|Tested Species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A 17 amino acid peptide from near the amino terminus of human ADAP.|
|Purification||Antigen affinity chromatography|
|Contains||0.02% sodium azide|
|Storage conditions||Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C|
A suggested positive control is K562 cell lysate.
PA5-20401 can be used with blocking peptide PEP-0518.
The adhesion and degranulation adaptor protein (ADAP) was initially identified as a molecular adapter that couples T cell receptor (TCR) stimulation to the avidity of integrins governing T cell adhesion. TCR stimulation promotes the formation of a multi-protein complex containing CARMA1, MALT1, and BCL-10, which through the association of ADAP, ultimately activates the NF-kappa-B family of transcription factors. More recent experiments have shown that ADAP controls optimal T cell proliferation, cytokine production, and expression of the Bcl-2 family member Bcl-x(L), suggesting that ADAP regulates T cell activation by promoting antigen-dependent T cell-antigen presenting cell (APC) activation. At least three isoforms of ADAP are known to exist.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: ADAP; ADAP, PRO0823, SLAP-130; Adhesion and degranulation promoting adaptor protein; adhesion and degranulation-promoting adaptor protein; FYB-120/130; FYN-binding protein; FYN-T-binding protein; p120/p130; SLAP-130; SLP-76-associated phosphoprotein
Gene Aliases: ADAP; FYB; PRO0823; SLAP-130; SLAP130
UniProt ID: (Human) O15117
Entrez Gene ID: (Human) 2533
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