|Flow Cytometry (Flow)||1/20|
|Immunocytochemistry (ICC)||Assay dependent|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1:500|
|Immunocytochemistry (ICC)||See 4 publications below|
|Western Blot (WB)||See 9 publications below|
|ELISA (ELISA)||See 1 publications below|
|Immunoprecipitation (IP)||See 1 publications below|
|Tested Species reactivity||Dog, Hamster, Human, Mouse, Rat|
|Published species reactivity||Rat , Non-human primate , Hamster , Bovine , Human , Mouse|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Recombinant human ARF1.|
|Contains||0.05% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
MA3-060 detects ADP-ribosylation factor1 (Arf1), Arf3, Arf5 and Arf6 and about ten-fold less well with Arf4 from human, mouse and rat tissues.
MA3-060 has been successfully used in Western blot, immunofluorescence, immunocytochemistry, and immunoprecipitation procedures. By Western blot, this antibody detects a single ~21 kDa protein representing Arf in rat pancreas extract. Immunofluorescence staining of Arf in rat pancreas with MA3-060 results in staining of the cytoplasmic face of the trans-Golgi membranes. MA3-060 cannot be used to neutralize Arf in solution.
The MA3-060 antigen is recombinant human Arf1.
The ADP-ribosylation factor (Arf) family comprises a group of structurally and functionally conserved 21 kDa proteins, which are members of the Ras superfamily of regulatory GTP-binding proteins. Arf is involved in intracellular protein traffic to and within the Golgi complex. Arf has a number of disparate activities including maintenance of organelle integrity, assembly of coat proteins, as a co-factor for cholera toxin and as an activator of phospholipase D. The Arf family is divided functionally into the Arf and the Arf-like (Arl) proteins. Arfs share more than 60% sequence identity, appear to be ubiquitous in eukaryotes, and are highly conserved evolutionarily.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: ADP-ribosylation factor 1; ADP-ribosylation factor 3; ADP-ribosylation factor 5; ADP-ribosylation factor 6; small GTP binding protein
Gene Aliases: 5430400P17Rik; AI788669; AI854770; ARF1; ARF3; ARF5; ARF6; AW496366
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