|Immunohistochemistry (Paraffin) (IHC (P))||1-2 ug/ml|
|Western Blot (WB)||1-2 ug/ml|
|Miscellaneous PubMed (MISC)||See 1 publications below|
|Immunofluorescence (IF)||See 1 publications below|
|Western Blot (WB)||See 1 publications below|
|Tested Species reactivity||Mouse|
|Published species reactivity||Rodent Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A peptide corresponding to amino acids 1805-1820 of Q9QZQ1.|
|Contains||0.09% sodium azide|
|Storage conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
This antibody is predicted to react with human, rat, chimpanzee, Rhesus monkey, canine, bovine and chicken based on sequence homology. Boiling samples prior to Western blot is not recommended.
ABfinity™ recombinant antibodies are rabbit monoclonal antibodies, unmatched for producing superior results. ABfinity™ antibodies are developed by immunizing animals, screening for functionality, cloning the immunogen-specific antibody genes into high-level mammalian expression vectors, produced on a large scale, and purified with Protein A.
ABfinity™ monoclonal antibodies resemble rabbit monoclonals isolated from serum or produced by hybridomas, but demonstrate greater specificity and sensitivity. Because ABfinity™ recombinant antibodies are derived from cloned DNA sequences of the heavy and light antibody chains, they are not susceptible to cell-line drift or lot-to-lot variation, thus allowing for peak specificity and performance.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
AF-6, also known as afadin, is expressed in a variety of cell types. At least 5 isoforms of this protein are produced by alternative splicing and the 'canonical' sequence is designated as l-afadin. Chromosomal aberration of the MLLT4 gene and tight junctions and Notch signaling pathways cross-communication. This happens through physical interaction with Ras, Notch and Dishevelled, a key Wingless effector. AF-6 also controls integrin-mediated cell adhesion. This is done at least partially by regulating Rap1 activation through recruitment of both SPA-1 and Rap1GTP. These results demonstrate that AF-6 is an important molecule mediating cell-cell adhesion but is also capable of modulating several key signaling pathways.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Afadin; Afadin adherens junction formation factor; MLLT4; myeloid/lymphoid or mixed lineage-leukemia translocation to 4 homolog; Protein Af-6
Gene Aliases: 5033403D15Rik; Af-6; AF6; Afadin; Afdn; Gm314; I-afadin; Mllt4; S-afadin
UniProt ID: (Mouse) Q9QZQ1
Entrez Gene ID: (Mouse) 17356
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