|Immunocytochemistry (ICC)||5-10 ug/ml|
|Immunofluorescence (IF)||Assay Dependent|
|Western Blot (WB)||2-5 ug/ml|
|Tested Species reactivity||Bovine, C. elegans, Human, Mouse, Rat|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Human heart mitochondria|
|Storage buffer||HEPES buffered saline|
|Contains||0.02% sodium azide|
|Storage conditions||4° C, do not freeze|
Apoptosis is characterized by several morphological nuclear changes including chromatin condensation and nuclear fragmentation. These changes are triggered by the activation of members of caspase family, caspase activated DNase, and several novel proteins. A novel gene, the product of which causes chromatin condensation and DNA fragmentation, was recently identified, cloned, and designated apoptosis inducing factor (AIF). Like the critical molecules, cytochrome c and caspase-9, in apoptosis, AIF localizes in mitochondria. AIF translocates to the nucleus when apoptosis is induced and induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. AIF induces chromatin condensation and DNA fragmentation, which are the hallmarks of apoptosis, of the isolated nucleus and the nucleus in live cells by microinjection. AIF is highly conserved between human and mouse and widely expressed.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: AIF; apoptosis-inducing factor 1; Apoptosis-inducing factor 1, mitochondrial; apoptosis-inducing factor, mitochondrion-associated, 1; COXPD6; harlequin; mitochondrial; PDCD8; programmed cell death 8 (apoptosis-inducing factor); Programmed cell death protein 8; striatal apoptosis-inducing factor; testicular secretory protein Li 4
Gene Aliases: AIF; AIFM1; AIFsh2; CMT2D; CMTX4; COWCK; COXPD6; DFNX5; Hq; NADMR; NAMSD; PDCD8
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