|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Western Blot (WB)||1-3 µg/ml|
|Tested Species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues 119-136 of human Akt3.|
|Storage buffer||PBS with 0.05% BSA|
|Contains||0.05% sodium azide|
|Storage conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
Suggested positive control: Hela whole cell extract, HeLa, antigen standard for AKT3 (transient overexpression lysate).
Akt, protein kinase B (PKB), is a serine/threonine kinase which is involved in many cellular signaling pathways and acts as a transducer of many functions initiated by growth factor receptors that activate phosphtidylinositol 3-kinase (PI 3-kinase). The major activity of Akt/PKB is to mediate cell survival. Akt/PKB is also believed to be a critical factor in the genesis of cancer as the tumor suppressor PTEN was found to antagonise PI-3 kinase and Akt/PKB kinase activity. There are 3 known isoforms of this enzyme in mammalian cells (1/alpha, 2/beta and 3/gamma). Akt1, 2 and 3 contain a key regulatory serine phosphorylation site in the carboxy-terminal region of the protein. Akt/PKB phosphorylation is critical for its activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: AKT3 kinase; DKFZp434N0250; PKB gamma; PKB-GAMMA; PKBG; PRKBG; Protein kinase Akt-3; Protein kinase B gamma; protein kinase B, gamma; RAC-gamma; RAC-gamma serine/threonine protein kinase; RAC-gamma serine/threonine-protein kinase; RAC-PK-gamma; STK-2; thymoma viral proto-oncogene 3; v-akt murine thymoma viral oncogene 3; v-akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma)
Gene Aliases: AI851531; AKT3; D930002M15Rik; MPPH; MPPH2; Nmf350; PKB-GAMMA; PKBG; PRKBG; RAC-gamma; RAC-PK-gamma; STK-2
Molecular Function: annexin calcium-binding protein calmodulin intracellular calcium-sensing protein kinase non-receptor serine/threonine protein kinase protein kinase transfer/carrier protein transferase
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