|Immunohistochemistry (IHC)||1:1000 - 1:5000|
|Western Blot (WB)||1:100 -1:10000|
|Tested Species reactivity||Human, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to the N-terminal residues C-K(11) K G E I F E L K A E L N S D K K E K(29) of human AP-1 complex subunit beta-1.|
|Storage buffer||whole serum|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
PA1-18307 detects AP-1 complex subunit beta-1 from human and rat samples.
PA1-18307 has been successfully used in immunohistochemistry (paraffin and frozen) and Western blot procedures. Antigen retrieval has been used in testing but may not be necessary.
The PA1-18307 immunogen is a synthetic peptide corresponding to the N-terminal residues C-K(11) K G E I F E L K A E L N S D K K E K(29) of human AP-1 complex subunit beta-1, conjugated to diphtheria toxin. The sequence is 100% conserved in mouse, rat and bovine.
Reconstitute with 100 ul of distilled water. Glycerol (1:1) may be added for additional stability.
AP-1 complex subunit beta-1 is a subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Adapter-related protein complex 1 gamma-1 subunit Gamma-adaptin; adapter-related protein complex 1 subunit beta-1; Adaptor protein complex AP-1 gamma-1 subunit; Adaptor protein complex AP-1 subunit beta-1; adaptor protein complex AP-1, beta 1 subunit; adaptor-related protein complex 1 beta 1 subunit; Adaptor-related protein complex 1 subunit beta-1; adaptor-related protein complex 1, beta 1 subunit; adaptor-related protein complex AP-1 beta 1 subunit; AP-1 complex subunit beta-1; Beta-1-adaptin; Beta-adaptin 1; beta-prime-adaptin; beta1-adaptin; Clathrin assembly protein complex 1 beta large chain; Golgi adaptor HA1/AP1 adaptin beta subunit; Golgi adaptor HA1/AP1 adaptin subunit g; plasma membrane adaptor HA2/AP2 adaptor beta subunit
Gene Aliases: ADTB1; AP105A; AP1B1; BAM22; CLAPB2
Molecular Function: membrane traffic protein
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.