|ChIP assay (ChIP)||Assay-Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:1000-1:10,000|
|Tested Species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 1 and 318 of Human APE1|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 10% glycerol|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
PA5-29157 targets APE1 in IF, IHC (P), and WB applications and shows reactivity with Human, Rat, and mouse samples.
The PA5-29157 immunogen is recombinant fragment corresponding to a region within amino acids 1 and 318 of Human APE1.
Apurinic/apyrimidinic (AP) sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. AP sites are pre-mutagenic lesions that can prevent normal DNA replication so the cell contains systems to identify and repair such sites. Class II AP endonucleases cleave the phosphodiester backbone 5' to the AP site. This gene encodes the major AP endonuclease in human cells. Splice variants have been found for this gene; all encode the same protein.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: AP endonuclease 1; AP endonuclease class I; AP lyase; APE1; APEN; APEX; APEX nuclease; APEX nuclease (multifunctional DNA repair enzyme) 1; APEX nuclease 1; Apurinic-apyrimidinic endonuclease 1; apurinic/apyrimidinic (abasic) endonuclease; apurinic/apyrimidinic endonuclease 1; deoxyribonuclease (apurinic or apyrimidinic); DNA-(apurinic or apyrimidinic site) lyase; HAP1; protein REF-1; Redox factor-1; REF-1; REF1
Gene Aliases: APE; APE1; APEN; APEX; APEX1; APX; HAP1; REF-1; REF1
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.