|Immunohistochemistry (IHC)||5 µg/ml|
|Western Blot (WB)||0.5-1 ug/ml|
|Tested Species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||An 18 amino acid peptide from near the center of human APH1.|
|Purification||Antigen affinity chromatography|
|Contains||0.02% sodium azide|
|Storage conditions||Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C|
Despite its predicted molecular weight, APH1 often migrates at aberrant locations in SDS-PAGE. A suggested positive control is human brain tissue lysate.
PA5-20318 can be used with blocking peptide PEP-0438.
APH1 was initially identified as a component of the Notch pathway in C. elegans. Along with nicastrin, PEN2, and presenilin-1 APH1 is an essential component of the -gamma-secretase complex which cleave the amyloid precursor protein (APP) at what are known as the -gamma- and epsilon-sites and can lead to the accumulation of the Amyloid beta peptide (Abeta) cleavage product that is associated with Alzheimer and quote;s disease. APH1 exists in at least three distinct isoforms with APH1a as the principal isoform present in the -gamma-secretase complex. Mice deficient in this isoform, but not the other two, were lethal at E10.5, with impaired vascular and neural development observed.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: anterior pharynx defective 1 homolog A; anterior pharynx defective 1a homolog; aph-1 homolog A, gamma secretase subunit; APH-1a; Aph-1alpha; APH1A gamma secretase subunit; Gamma-secretase subunit APH-1A; Presenilin-stabilization factor; RP4-790G17.3, 6530402N02Rik, APH-1, APH-1A, CGI-78
Gene Aliases: 6530402N02Rik; APH-1; APH-1A; APH1A; CGI-78; PSF; RGD1311546; UNQ579/PRO1141
Molecular Function: enzyme modulator
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