|Western Blot (WB)||1:1000|
|Tested Species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||KLH conjugated synthetic peptide between 61-90 amino acids from human ASK1(MEKK5/MAP3K5)|
|Purification||Antigen affinity chromatography|
|Contains||0.09% sodium azide|
|Storage conditions||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C|
Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. MAPKKK5 contains 1,374 amino acids with all 11 kinase subdomains. Northern blot analysis shows that MAPKKK5 transcript is abundantly expressed in human heart and pancreas. The MAPKKK5 protein phosphorylates and activates MKK4 (aliases SERK1, MAPKK4) in vitro, and activates c-Jun N-terminal kinase (JNK)/stress-activated protein kinase (SAPK) during transient expression in COS and 293 cells; MAPKKK5 does not activate MAPK/ERK.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: apoptosis signal regulating kinase 1; apoptosis signal-regulating kinase 1; ASK-1; MAP/ERK kinase kinase 5; MAP3K5; MAPK/ERK kinase kinase 5; MEK kinase 5; MEKK 5
Gene Aliases: ASK1; MAPKKK5; MEKK5
UniProt ID: (Human) Q99683
Entrez Gene ID: (Human) 4217
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